Fig. 1.

Prostratin stimulates productive FIV infection from infected, IL-2-depleted T-cells. (A) IL-2 depleted, FIV GL8-infected (M.O.I. = 0.01) MYA-1 CD4⁺ T-cells were stimulated with either PMA, Prostratin (Pro) or mock stimulated (DMSO) 4 days after infection and virus production was measured by FIV p24 ELISA. (B) DNA was extracted from FIV infected cells at day 12 post infection and screened by PCR for either a 466 bp FIV LTR–gag product or a GAPDH control. Lanes: (1) −FIV, +IL2; (2) +FIV, +IL-2; (3) +FIV, −IL-2, +DMSO; (4) +FIV, −IL-2, +PMA; (5) +FIV −IL-2, +Prostratin, (6) FIV plasmid positive control, (7) dH₂O negative control.