Fig. 2.

De novo infection contributes to most of the virus production detected by our assay. MYA-1 T-cells supplemented with or depleted of IL-2 for 24 h were infected with FIV GL8 (M.O.I. = 0.01). Virus production was measured using (A) FIV p24 ELISA and (B) qPCR. The antiretrovirals RGV and AZT were added to appropriate samples 24 h after infection. Each point represents mean ± standard error for the p24 ELISA data and mean ± standard deviation (n = 3) of the calculated copy number for the qPCR data and representative of two independent experiments. (C) IL-2-depleted MYA-1 CD4⁺ T-cells were infected with FIV GL8 (M.O.I. = 0.06). Two days after infection the cells were stimulated with PMA, Prostratin (Pro) or mock stimulated (DMSO). Supernatant was collected at day 10 post-infection and virus production was quantified by a reverse transcriptase (RT) activity assay. (D) Parallel experiment to (C) in which RGV was added to the FIV-infected cells 24 h post-infection and 24 h prior to treatment with PMA or Prostratin. Each bar represents the mean ± standard error (n = 3) and representative of three independent experiments.