Fig. 4.

IL-2 or Prostratin-driven FIV replication requires PKC. IL-2-depleted MYA-1 CD4⁺ T-cells were infected with FIV GL8 (M.O.I. = 0.01). Two days post-infection, cells were stimulated with Prostratin (Pro), Prostratin plus Gö6850 (Pro + Gö6850), IL-2 (100 U/ml), IL-2 + Gö6850, Gö6850 or mocked stimulated (DMSO). Control uninfected cells were maintained in the absence of IL-2 (−FIV) and (A) virus production was monitored by a reverse transcriptase (RT) activity assay. (B) Viability of the cells receiving each treatment (as in (A)) in the presence or absence of FIV was measured at 8 days post-infection by Trypan Blue exclusion. Each bar represents the mean ± standard error (n = 3) and results are representative of three independent experiments. (p.i.) indicates IL-2 was added post infection (*p < 0.05; **p < 0.01).