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. 2013 Jan;171(1):121–128. doi: 10.1016/j.virusres.2012.11.004

Fig. 5.

Fig. 5

Inhibition of productive infection with FIV by Prostratin in the presence of IL-2 is PKC-dependent. MYA-1 CD4+ T-cells were cultured in the presence of IL-2 and infected with FIV GL8 (M.O.I. = 0.01). Prostratin (Pro), Prostratin and Gö6850 (Pro + Gö6850), Gö6850, or solvent (DMSO) were added to the cells 2 days post-infection. At day 8 post-infection, (A) virus production was quantified in the culture supernatant by a reverse transcriptase (RT) activity assay while (B) the viability of the infected and un-infected cells was assessed by Trypan Blue exclusion. Each bar represents the mean ± standard error (n = 3) and is representative of two independent experiments. In separate experiments, virus production was quantified at day 8 post-infection by FIV p24 ELISA (C) and the viability of FIV infected cells were assessed by Trypan Blue exclusion (D). Each bar represents the mean ± standard error (n = 4) and the data are the combination of two experiments (*p < 0.05; **p < 0.01).