Fig. 7.

No reduction in intracellular FIV DNA following pre-treatment of CD4⁺ T-cells with Prostratin. IL-2 supplemented MYA-1 CD4⁺ T-cells were pre-incubated with Prostratin (Pro), Prostratin plus Gö6850, Gö6850 alone or mocked treated (DMSO) for 24 h before infection with FIV GL8. 2 h post infection the cells were washed twice and total cellular DNA extracted. qPCR amplifying a region of the FIV gag gene was performed on the DNA samples and the results were analysed using the ΔΔCt method. The data were relative to the average ΔCt value of the mock treated (DMSO) cells. Each bar represents the mean ± standard deviation of the calculated relative quantities (n = 3) and is representative of three independent experiments.