Table III. Ag-specific rat IgG hybridomas with fully human idiotypes.
Animal | Ag | Cellsa | Fusions | Titer | Hybrids | IgGsb | KD (nM)c |
---|---|---|---|---|---|---|---|
SD | PGRN | LN | 1 | 38,400 | 3,520 | 38 | 0.3–1.0 |
OmniRat | PGRN | LN | 1 | 12,800 | 1,600 | 148 | 0.7–2.4 |
SD | PGRN | SP | 1 | 51,200 | 8,000 | 29 | ND |
OmniRat | PGRN | SP | 1 | 51,200 | 36,000 | 24 | ND |
OmniRat | hGHR | LN | 3 | 4,800 | 704–1,024 | 18, 3, 2 | ND |
SD | hGHR | SP | 1 | 204,800 | 53,760 | 230 | <0.07–0.4 |
OmniRat | hGHR | SP | 1 | 76,800 | 53,760 | 7 | 0.16–2.4 |
OmniRat | CD14 | SP | 2 | 102,400 | 2,800–3500 | 54, 14 | <0.1–0.2 |
SD | TAU/KLH | LN | 1 | 20,000 | 1,728 | 99d | 0.6–2.4 |
OmniRat | TAU/KLH | LN | 1 | 4,800 | 1,880 | 118d | 0.5–3.2 |
SD | HEL | LN | 1 | 12,800 | 1,564 | 26 | 0.02–0.1 |
OmniRat | HEL | LN | 3 | 25,600 | 288–640 | 0, 2, 7 | 0.6–1.5 |
SD | HEL | SP | 1 | 6,400 | 30,720 | 0 | ND |
SD | OVA | LN | 1 | 9,600 | 1,488 | 10 | 1.1–4.8 |
OmniRat | OVA | LN | 4 | 8,000 | 512–2240 | 0, 30, 0, 1 | 0.7–1.5 |
OmniRat animals (HC14/Huκ and/or Huλ/JKOJKO/KKOKKO) and control SD rats were immunized with human PGRN, hGHR, human CD14, TAU peptide (TAU/KLH), HEL, or OVA. LN, lymph node; SP, spleen cell.
Cell numbers were 3–9 × 107/fusion.
Ag specificity confirmed by biosensor analysis.
Range of five highest affinities.
Eight mAbs were specific for Tau peptide.