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. 2012 Dec 13;304(4):G322–G327. doi: 10.1152/ajpgi.00263.2012

Fig. 4.

Fig. 4.

Bile acid taurodeoxycholic acid (TDCA)-induced cell proliferation in AGS cells. A: TDCA treatment significantly increased thymidine incorporation in AGS cells. Knockdown of TGR5 with TGR5 small-interfering RNA (siRNA) significantly decreased thymidine incorporation at the basal condition as well as in response to TDCA treatment. B: a typical image of three Western blot analyses. C: summarized data showing that transfection of AGS cells with TGR5 plasmid significantly increased TGR5 protein expression. D: TDCA treatment significantly increased thymidine incorporation in AGS cells. This increase was significantly enhanced by overexpression of TGR5; n = 3 experiments. ANOVA: #P < 0.05 compared with the control group treated with control siRNA; ##P < 0.01 compared with the TDCA group treated with control siRNA; *P < 0.05 compared with the control group transfected with pcDNA3.1; **P < 0.05 compared with the TDCA group transfected with pcDNA3.1.