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. 2012 Dec 20;304(4):G371–G380. doi: 10.1152/ajpgi.00400.2012

Fig. 3.

Fig. 3.

Bile acid-mediated activation of TGR5 initiates a novel cAMP signaling cascade. Real-time analysis of TGR5 activation (cAMP signaling) in TGR5-bioluminescence resonance energy transfer (BRET) cells after treatment with the indicated concentrations of bile acid (BA) (A) or isoproterenol (B). C: Real-time analysis of TGR5 activation (cAMP signaling) in TGR5-BRET cells after treatment with bile acid [3, 10, and 30 μM taurocholic acid (TCA)], Colesevelam (Col) alone, oleic acid alone, or bound Col/TCA equivalent to 55 μM (see materials and methods). Arrow indicates when factors were added to cells. GCA, glycocholic acid; TCDCA, taurine chenoxycholic acid; GCDCA, glycine chenoxycholic acid.