Figure 2. Flow cytometry assay performed using splenocytes isolated from the mice at day 10 after the i.n. C. trachomatis challenge.

A. CD4⁺CD44^high splenic T-cells were gated, and the frequency of positive intracellular IFN-γ staining in CD4⁺CD44^high splenic T-cells was exposed.

B. CD8⁺CD44^high splenic T-cells were gated, and the frequency of positive intracellular IFN-γ staining in CD8⁺CD44^high splenic T-cells was exposed.

C. CD4⁺CD44^high splenic T-cells were gated, and the frequency of positive intracellular IL-4 staining in CD4⁺CD44^high splenic T-cells was exposed.

D. CD8⁺CD44^high splenic T-cells were gated, and the frequency of positive intracellular IL-4 staining in CD8⁺CD44^high splenic T-cells was exposed.