Figure 4.

Enzyme histochemical detection of acid phosphatase activity. A, Cryosection of wild-type vallate papilla, developed to reveal enzymatic conversion of AMP to adenosine at pH 5.6. The enzymatic reaction conditions are optimized to detect ACPP. Only faint signal is detectable throughout the tissue with the exception of von Ebner glands located deep in the tissue (arrowhead). B, At higher magnification, reaction product is detected at low levels in the taste bud although individual cells cannot readily be distinguished. C, Von Ebner glands below taste epithelium show prominent signal in acini. D, Dorsal root ganglion from the same wild-type mouse as in A shows some small and medium sized neurons are positive for the acid phosphatase, while large neurons and the epineurium are negative. Scale bars: A, 100 μm; B, 20 μm; C, D, 25 μm.