Figure 3. Weight loss and arthritis development after S. aureus LS-1 i.v. injection in Ncf1 mutant and wt mice.
5.7×107 S. aureus LS-1 was injected i.v. in Ncf1 wt and mutant mice (n=19 mice per group). Arthritis severity and incidence (A and B) and weight (C) were evaluated over 14 days. (D) Four mice per group were sacrificed on day 3, 7, and 10, and the remaining 7 mice per group were sacrificed on day 14 after infection, and quantitative bacterial cultures were performed on kidneys. Mean and SEM is shown, except for incidence. * P<0.05, ** P<0.01. Statistics was calculated with Mann-Whitney for arthritis score and weight and with Kruskal-Wallis with Dunn’s comparison post-test for bacterial cultures. On fig 3 A–C only the 7 mice/genotype that were kept alive until day 14 are illustrated. (E–F) Histology of healthy and affected paw after injection of S. aureus LS-1, respectively. 5× magnification.