Figure 5. NCF1 expression in monocytes protects from Staphylococci.
0.8×108 bacteria of S. xylosus 720 isolated from infected paws of Ncf1 mutant mice were injected i.v. into Ncf1*/* MN+/− and non transgenic littermates Ncf1*/* mice as well as Ncf1 wt (Ncf1+/+) mice (N=6–9). Survival (A) and weight change (B) were monitored over 7 days. Survival curves were displayed by Kaplan-Meier and intergroup comparisons assessed by the log-rank method: *** P<0.001. For weight changes, mean and SEM is shown and genotypes were compared using Mann-Whitney *P<0.05.
(C) Male Ncf1*/* MN+/− mice (N=9), as well as littermates negative for the transgene (Ncf1*/*, N=10) and Ncf1 wt (Ncf1+/+) (N=7) mice were injected i.v. with 5.7 × 10^7 S. aureus LS-1. Weight was monitored for 7 days. Mean and SEM is shown. * P<0.05 and ** P<0.01 between Ncf1*/* and Ncf1 +/+ mice, # P<0.05 between Ncf1*/* MN+/− and Ncf1*/* calculated with Kruskal-Wallis with Dunn’s comparison post-test.