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. Author manuscript; available in PMC: 2013 Feb 7.
Published in final edited form as: J Microsc. 2011 Mar 1;243(2):154–171. doi: 10.1111/j.1365-2818.2011.03489.x

Figure 2.

Figure 2

Illustrating preliminary estimation of the lateral offset for a pair of 5-channel images of the cortical surface (blue: nuclei, purple: Nissl, Green: microvasculature, yellow: microglia, red: astrocytes). Panels (A) and (B) show the maximum-intensity projections of the two adjacent optical stacks with all 5 channels overlaid. Panels (C) and (D) show the generic landmarks for these images overlaid on the fusion image derived by combining the 5-channel data into one. The yellow circles indicate corners and the yellow lines indicate the locations and normal directions of edge points. Panel (E) shows the alignment produced by the GDB-ICP pair-wise registration of the projection. The transformation computed from panels (C) and (D) was applied to the 5-color projections, and used to construct a 2-D montage of these two projection images. Images in this figure are taken from data set #2.