FIG. 2.

One-layered (1L) poly(glycerol sebacate) (PGS) scaffolds with precisely defined, anisotropic pores were rapidly and reproducibly micromolded from silicon masters. Representative light micrographs (A–C) of etched silicon molds demonstrating three scaffold designs: 2:1 CR (A, D, G, H, I), 5:1 CR (B, E, J, K, L), and 2:1 HR (C, F). Scanning electron micrographs of PGS scaffolds delaminated from silicon molds illustrate successful pattern replication (D–F) and full-thickness scaffolds from 50-μm deep (G, J,), 100-μm deep (H, K), and 150-μm deep molds (I, L), viewed from above (D–F) or 45 degrees (G–L). Scaffolds were fabricated either with through pores (G, J, I, L), or with an ∼50-μm-thick PGS film at the base for enhanced cell retention (H, K), indicated by black arrowheads. Scale bars: 200 μm.