Figure 2.

Adenosine A1R signaling is elevated by 12 h of sleep deprivation. (a) Representative field excitatory postsynaptic potentials (fEPSP) measured at the Schaeffer collateral synapse before (black) or following (red) the application of 8-cyclopentyltheophylline (CPT, 200 nℳ). (b) Adenosine tone increases following wakefulness (ZT=0) and declines during sleep (ZT=12) in slices from wildtype but not dnSNARE or A1R knockout (A1R^−/−) mice. Transient sleep deprivation (12 h s.d.) maintains the wakefulness-dependent increase in adenosine tone that is impaired by astrocytic dnSNARE. **P⩽0.01, ***P⩽0.001.