Table 1. Primers for Polymerase Chain Reaction Amplification.
No | Gene | SNP | Rs No. | Primer name | marker | Sequence |
---|---|---|---|---|---|---|
1 |
Endothelin-1 |
G5665T |
rs5370 |
F(Gtype) |
FITC |
CCAAGCTGAAAGGCAXGC |
F(Ttype) |
TxR |
CCCAAGCTGAAAGGCAXTC |
||||
R |
Biotin |
TCACATAACGCTCTCTGGAGG |
||||
2 |
ACEI/D |
I/D |
rs1799752 |
F(Itype) |
FITC |
CTCGATCTCCTGACCTCGTGATCC |
F(Dtype) |
TxR |
CCTGCTGCCTATACAGTCACTTTTATG |
||||
R |
Biotin |
GATGTGGCCATCACATTCGTCAGAT |
||||
3 |
MTHFR |
C677T |
rs1801133 |
F(Ttype) |
FITC |
GAGAAGGTGTCTGCGGGAXTC |
F(Ctype) |
TxR |
GAAGGTGTCTGCGGGAXCC |
||||
R | Biotin | GAATGTGTCAGCCTCAAAGAAA |