Table 1. Numbers of total crystallin spectral counts (an indication of relative crystallin abundance), cysteine-containing peptide spectral counts, number of –SH groups for each crystallin, and numbers of –SH groups bound by GSH or cysteine for each guinea pig lens water-soluble nuclear crystallin.
| Crystallin* | Total spectral counts** | peptide counts as % of total | Cysteine-containing peptide counts** | Cysteine-peptide counts as % of total | Number of -SH groups per crystallin | -SH groups bound by GSH or cysteine*** |
|---|---|---|---|---|---|---|
| ζ |
4500 |
15.9 |
828 |
10.4 |
5 |
4 |
| γS |
2947 |
10.4 |
695 |
8.7 |
7 |
5 |
| βB2 |
2794 |
9.9 |
516 |
6.4 |
2 |
2 |
| αA |
2722 |
9.7 |
510 |
6.4 |
1 |
1 |
| γB |
2664 |
9.4 |
1240 |
15.5 |
7 |
5 |
| βB1 |
2299 |
8.2 |
865 |
10.8 |
5 |
4 |
| γC |
2226 |
7.9 |
680 |
8.5 |
7 |
5 |
| βA4 |
1874 |
6.6 |
479 |
6 |
4 |
4 |
| βA1/A3 |
1757 |
6.2 |
1173 |
14.7 |
8 |
6 |
| αB |
1597 |
5.7 |
0 |
0 |
0 |
0 |
| βB3 |
1306 |
4.6 |
159 |
2 |
3 |
2 |
| γA |
922 |
3.3 |
709 |
8.9 |
7 |
4 |
| βA2 |
613 |
2.2 |
140 |
1.7 |
5 |
2 |
| total | 28221 | 100 | 7994 | 100 | 61 | 44 |
* γN-crystallin was not included since it had very low numbers (<50) of MS/MS spectral counts. **Averages of control and hyperbaric oxygen-treated results. *** The number of –SH groups in either control or hyperbaric oxygen-treated samples that were found to bind either glutathione or cysteine.