Skip to main content
. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2013 Jan 15;73(2):662–671. doi: 10.1158/0008-5472.CAN-12-0653

Figure 3. CCL2 mediates Twist1-induced angiogenesis in the CAM assay.

Figure 3

A) QPCR analysis of CCL2 mRNA levels in HMLE-Ctrl and HMLE-Twist1 cells with indicated shRNAs. B) The levels of CCL2 protein were measured by ELISA in conditioned media from indicated HMLE cells. C) Lysates from indicated HMLE cells were analyzed by SDS-PAGE and probed for Twist1, E-cadherin, fibronectin, and α-tubulin (full-length blots, SFig.3A). D) Quantification of angiogenesis from three independent CAM angiogenesis experiments. For each experiment, 5–8 onplants were evenly distributed across 4–6 ten-day-old embryos per cell type tested, *p<0.05 versus HMLE-Ctrl, #p<0.05 versus HMLE-Twist1 shCtrl by ANOVA followed by Tukey Test.