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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Cancer Res. 2013 Jan 15;73(2):662–671. doi: 10.1158/0008-5472.CAN-12-0653

Figure 4. Twist1 expression in mammary epithelial cells promotes macrophage attraction in a CCL2-dependent manner.

Figure 4

A) Cell surface expression of CCR2 on isolated mouse BMDM as measured by flow cytometry analysis using anti-CCR2 (open) versus Control IgG (shaded) antibody staining. B) BMDM were subjected to Boyden Chamber migration assays toward conditioned media from HMLE-Ctrl or HMLE-Twist1 cells. Representative images of migrated macrophages stained with crystal violet (top) and quantification of relative migration (bottom) for one representative experiment, *p<0.05 by Student’s T-test. C) Macrophage migration toward conditioned media from 168FARN cells with indicated shRNAs for one representative experiment, *p<0.05 versus shCtrl by ANOVA followed by Tukey Test. D) Macrophage migration toward conditioned media from 168FARN cells with shRNAs against Twist1, coincubated with either PBS or rCCL2 (10 ng/mL) for one representative experiment, *p<0.05 by Student’s T-test. E) Macrophage migration toward conditioned media from HMLE-Twist1 cells expressing indicated shRNAs for one representative experiment, *p<0.05 versus shCtrl by ANOVA followed by Tukey Test.