Figure 4. Phenotypic complementation of ΔpfkAΔpfkB E. coli mutant with mycobacterial pfkA and pfkB.

(i) Δ pfkAΔpfkB E. coli RL257, (ii) RL257 complemented with Mtb pfkA, (iii) RL257 complemented with Mtb pfkB and (iv) RL257 transformed with empty vector were grown on M9 minimal agar supplemented with (A) 0.2% glucose, (B) 0.2% glucose and IPTG and (C) glycerol.