Figure 5. In vitro growth kinetic of ΔpfkA and ΔpfkB mutants on various carbon sources.

Growth in liquid medium with glucose, glycerol or acetate as sole carbon source (as indicated) was monitored for Wild-type, ΔpfkA, ΔpfkA complemented with pfkA, ΔpfkA complemented with pfkB, and Δ pfkB M. tuberculosis strains (as indicated). Bacterial growth was monitored by OD absorbance at 600 nm over time. Results are representative of at least two independent experiments.