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. 2013 Feb 7;8(2):e56291. doi: 10.1371/journal.pone.0056291

Figure 6. Immuno-detection of TAK1 and CagA in α-CagA or α-TAK1 respective immuno-precipitated lysates derived from AGS cells infected with H.

Figure 6

pylori CagA mutant strains, at 1 hour post infection. (Panel A) Immunoprecipitation utilizing polyclonal α-CagA antibody (Austral Biologicals) and TAK1 western blot immunodetection utilizing monoclonal α-TAK1 antibody. Immunoprecipitation utilizing monoclonal α-TAK1 antibody and CagA immunodetection utilizing an α-CagA monoclonal antibody raised against the C-terminal (Panel B) or the N-terminal (Panel C) end of CagA protein. Note that the α-CagA monoclonal antibody raised against the C-terminal (Austral Biologicals) maps exactly on the EPIYA-C domains and fails to detect the P12AB CagA variant in panel B.