Figure 4. Cytofluorimetric analysis of GFP expression in blood cells from WT and thy1GFP-M mice.

(A) GFP expression was evaluated in monocytes as CD11b positive, GR-1 (Ly6C, Ly6G) intermediate, F4/80 positive cells (CD11b⁺ GR1^int F4/80⁺); in granulocytes as CD11b positive, GR-1 highly positive cells (CD11b⁺ GR1^high); in dendritic cells as CD11b positive, GR-1 negative, F4/80 negative, CD11c positive cells (CD11b⁺ GR1^- F4/80^- CD11c⁺). (B) Lymphocyte population were phenotypically characteryzed as B220 positive, CD3 negative cells (B lymphocytes, B220⁺ CD3^-); B220 negative, CD3 positive, CD8 positive, CD4 negative cells (T cytotoxic cells, B220^- CD3⁺ CD8⁺ CD4^-) and B220 negative, CD3 positive, CD8 negative, CD4 positive (T helper, B220^- CD3⁺ CD8^- CD4⁺). Percentages inside the gates represent positive cells of parent populations. The figures shown are a representative experiment of 3 other separate experiments from 3 different mice performed with similar results.