Table 1. Species-specific primers used to identify ten different bacterial species in plaque samples of experimental gingivitis at day 21 of the study.

Bacterial species	Primer	Annealing temperature	Expected amplicon size (bp)	Reference
Prevotella intermedia	F: 5′-CGTGGACCAAAGATTCATCGGTGGA-3′	64°C	259	[40]
	R: 5′-CCGCTTTACTCCCCAACAAA-3′
Fusobacterium nucleatum	F: 5′-AGAGTTTGATCCTGGCTCAG-3′	60°C	360	[41]
	R: 5′-GTCATCGTGCACACAGAATTGCTG-3′
Streptococcus oralis	F: 5′-TCCCGGTCAGCAAACTCCAGCC-3′	66°C	374	[42]
	R: 5′-GCAACCTTTGGATTTGCAAC-3′
Streptococcus sanguinis	F: 5′-GGATAGTGGCTCAGGGCAGCCAGTT-3′	70°C	313	[42]
	R: 5′-GAACAGTTGCTGGACTTGCTTGTC-3′
Capnocytophaga sputigena	F: 5′-AGAGTTTGATCCTGGCTCAG-3′	55°C	185	[43]
	R: 5′-GATGCCGCTCCTATATACCATTAGG-3′
Eikenella corrodens	F: 5′-CTAATACCGCATACGTCCTAAG-3′	60°C	688	[44]
	R: 5′-CTACTAAGCAATCAAGTTGCCC-3′
Tannerella forsythia	F: 5′-GCGTATGTAACCTGCCCGCA-3′	60°C	641	[44]
	R: 5′-TGCTTCAGTGTCAGTTATACCT-3′
Porphyromonas gingivalis	F: 5′-AGGCAGCTTGCCATACTGCG-3′	60°C	404	[44]
	R: 5′-ACTGTTAGCAACTACCGATGT-3′
Treponema denticola	F: 5′-TAATACCGAATGTGCTCATTTACAT-3′	60°C	316	[44]
	R: 5′-TCAAAGAAGCATTCCCTCTTCTTCTTA-3′
Veillonella parvula	F: 5′-GAAGCATTGGAAGCGAAAGTTTCG-3′	57°C	623	[45]
	R: 5′-GTGTAACAAGGGAGTACGGACC-3′
Aggregatibacter actinomycetemcomitans	F: 5′-TAGCCCTGGTGCCCGAAGC-3′	68°C	557	[46]
	R: 5′-CATCGCTGGTTGGTTACCCTCTG-3′