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. 2013 Feb 7;8(2):e56484. doi: 10.1371/journal.pone.0056484

Figure 6. Co-culture of Schwann cells with NG108-15 neurons.

Figure 6

(A) NG108-15 cells were either grown alone or on top of a monolayer of Schwann cells isolated from control nerve or distal nerve segments taken from animals undergoing immediate (0) or 6 month delayed nerve repair (6). Cultures were stained with βIII tubulin antibody (green) and DAPI (blue). (B) Quantitative analysis of neurite outgrowth was performed on NG108-15 neurons grown in the absence (control) or presence of Schwann cells isolated from control nerve (+SC) or distal nerve segments from animals undergoing nerve repair immediately following injury (0) or delayed repair at 1, 3, 6 months. ***P<0.001 significantly different from values in the absence of Schwann cells. There was no significant difference between any of the cultures in the presence of Schwann cells.