Table 1. Primer sequences for RT-PCR and annealing temperatures (°C).
Factor | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | °C |
S100B | GTTGCCCTCATTGATGTCTTC | AGACGAAGGCCATAAACTCCT | 57.9 |
erbB2 | AACCTTTCCTTGCTGCTTGA | GTTCCCTCCAGACCTCTTCC | 59.9 |
erbB3 | AGAGGCTTGTCTGGATTCT | AGGAGTAAGCAGGCTGTGT | 55.9 |
erbB4 | AACCAGCACCATACCAGAGG | TTCATCCAGTTCTGCTCGTG | 62.1 |
TGF-β | CTAATGGTGGACCGCAACAAC | CGGTTCATGTCATGGATGGTG | 67.8 |
tenascin C | GCCTCAACAACTGCTACAATCGTG | TCAGCCCCTGTGAACCCATC | 66.1 |
collagen I | GTGAACCTGGCAAACAAGGT | CTGGAGACCAGAGAAGCCAC | 64.1 |
phosphacan | GAATTCTGGTCCACCAGCAG | GGTTTATACTGCCCTCTTTAGG | 59.0 |
versican | ACACAGGGAGAAACCCAGGA | TGTCTTGTTTTCTCTGACCT | 61.0 |
α-nAChR | TGTGTCTCATCGGGACGC | GGGCAGAGGGAGGCTTAGTTC | 64.0 |
β-nAChR | CCGTGTCACTGCTGAATCTGT | CTCAAAGGACACCACGACAT | 60.9 |
γ-nAChR | TGTCATCAACATCATCGTCCC | CGAGGAAAAGGAAGACGGT | 61.8 |
δ-nAChR | TGTGGAGAGAAGACCTCG | AGCCTCTTGGAGATAAGCAAC | 57.0 |
ε-nAChR | AACTGTCTGACTGGGTGCGT | GAAGATGAGCGTAGAACCGAC | 61.8 |
MuSK | TGAAGCTGGAAGTGGAGGTTTT | GCAGCGTAGGGTTACAAAGGAA | 63.3 |
18S | TCAACTTTCGATGGTAGTCGC | CCTCCAATGGATCCTCGTTAA | 62.1 |
actin | ACTATCGGCAATGAGCGGTTC | AGAGCCACCAATCCACACAGA | 64.1 |