Table 1. Primer sequences for RT-PCR and annealing temperatures (°C).

Factor	Forward Primer (5′→3′)	Reverse Primer (5′→3′)	°C
S100B	GTTGCCCTCATTGATGTCTTC	AGACGAAGGCCATAAACTCCT	57.9
erbB2	AACCTTTCCTTGCTGCTTGA	GTTCCCTCCAGACCTCTTCC	59.9
erbB3	AGAGGCTTGTCTGGATTCT	AGGAGTAAGCAGGCTGTGT	55.9
erbB4	AACCAGCACCATACCAGAGG	TTCATCCAGTTCTGCTCGTG	62.1
TGF-β	CTAATGGTGGACCGCAACAAC	CGGTTCATGTCATGGATGGTG	67.8
tenascin C	GCCTCAACAACTGCTACAATCGTG	TCAGCCCCTGTGAACCCATC	66.1
collagen I	GTGAACCTGGCAAACAAGGT	CTGGAGACCAGAGAAGCCAC	64.1
phosphacan	GAATTCTGGTCCACCAGCAG	GGTTTATACTGCCCTCTTTAGG	59.0
versican	ACACAGGGAGAAACCCAGGA	TGTCTTGTTTTCTCTGACCT	61.0
α-nAChR	TGTGTCTCATCGGGACGC	GGGCAGAGGGAGGCTTAGTTC	64.0
β-nAChR	CCGTGTCACTGCTGAATCTGT	CTCAAAGGACACCACGACAT	60.9
γ-nAChR	TGTCATCAACATCATCGTCCC	CGAGGAAAAGGAAGACGGT	61.8
δ-nAChR	TGTGGAGAGAAGACCTCG	AGCCTCTTGGAGATAAGCAAC	57.0
ε-nAChR	AACTGTCTGACTGGGTGCGT	GAAGATGAGCGTAGAACCGAC	61.8
MuSK	TGAAGCTGGAAGTGGAGGTTTT	GCAGCGTAGGGTTACAAAGGAA	63.3
18S	TCAACTTTCGATGGTAGTCGC	CCTCCAATGGATCCTCGTTAA	62.1
actin	ACTATCGGCAATGAGCGGTTC	AGAGCCACCAATCCACACAGA	64.1