Table 1. Total phenolic content and evaluation of the free radical scavenging activity of EE96 and eluates EL30–EL70.
Eluate | Total phenolic content (g GAE/L) | Free radical scavenging activity (g GAE/L) |
EE96 | 1.99±0.01a | 1.88±0.01a |
EL30 | 0.26±0.01d | 0.18±0.08d |
EL40 | 0.39±0.01c | 0.33±0.08c |
EL50 | 0.26±0.01d | 0.30±0.05c |
EL60 | 0.82±0.01b | 1.20±0.03b |
EL70 | 0.83±0.02b | 1.22±0.02b |
Data are means ±S.D. (n = 3); values in the same column followed by the same letter (a-d) are not statistically different (P<0.05), as measured by Duncan’s test.
EE96, ethanolic extract of crude propolis extract prepared using SPE with 96% ethanol elution.
EL30-EL70, eluates of the crude propolis extract prepared using SPE with 30% to 70% ethanol elution.
GAE, gallic acid equivalent.