Figure 7. Developmental expression of Lstra.

Total RNA was extracted from L. sericata mixed sex embryos and pupae and adult male and female. The embryos were collected within one hour of laying then aged for the indicated period at room temperature. (A) RT-PCR with Lstra primers yielded a 1.1 kb male product and 0.8 kb female product as indicated. The position of the primer pair used for RT-PCR is shown in Figure 1. (B) RT-PCR product of the cellularization gene Lsslam and (C) Lsmsl2 as a loading control.