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. 2013 Feb 7;8(2):e55753. doi: 10.1371/journal.pone.0055753

Figure 5. Effect of AZT and LAC treatments on hippocampal AMPA and NMDA receptors expression.

Figure 5

(A) Representatives of immunoblots with GluR1 and GluR2 receptor antibodies showed a 110 kDa and 100 kDa bands corresponding to AMPA receptor subunits, respectively. Blot with NR1 receptor antibody showed a 130 kDa band, corresponding to NMDA receptor subunit. (B) Results are expressed as the ratio of the optical density (OD) of the GluR1, GluR2 and NR1 band and the β-actin band. AZT prenatal treatment caused a significant reduction of the GluR1 subunit of AMPA receptors expression in respect to Control mice (* p<0.05 AZT vs Control, Student's t-test) that was fully prevented by LAC treatment: in AZT+LAC mice the Glu1 subunit was increased in respect to AZT mice (# p<0.05 vs AZT, Student's t-test) and did not differ from Controls. Expression of the GluR2 subunit of AMPA and NR1 subunit of NMDA receptors was not affected by either treatment. Values are expressed as means ± S.E.M. (n = 6 mice per group).