Figure 2. Mtd sequences and selections.
(A) The mutable residues of clones recombinantly expressed as GST fusion proteins. The lysozyme-binding L-Mtd variant is indicated by the asterisk. Shading identifies altered residues based on the wild-type Mtd sequence. Analysis of sequences from the (B) naïve Bvg–-SMPL library and sequences from (C) selections targeting lysozyme. The bar graph indicates the occurrence of tyrosine residues amongst the Bvg–-SMPL sequences. The line graph indicates mean hydrophobicity scores for the Bvg–-SMPL sequences relative to wild-type residues. Error bars represent standard deviation (n = 36).
