Figure 4. The rate of change of the growth over time (OD₆₀₀/Hrs) of E. coli strains containing various alanine aminotransferase enzymes.

The smooth first derivative of each time trial in Figure 4 was determined, indicating the change in the growth rate of the bacteria containing each of the different AlaATs and the controls over time. E. coli expressing AtAlaAT2, MtAlaAT, ScAlaAT1 and MmAlaAT1 showed changes in the rate of growth over the 26 hour time period similar to that seen by Arabidopsis thaliana AlaAT1 expressing cells.