Table 1. Summary of kinetic assay results.
| Enzyme | Alanine | 2-oxoglutarate | Glutamate | Pyruvate | ||||
| KM (mM) | SE KM | KM (mM) | SE KM | KM (mM) | SE KM | KM (mM) | SE KM | |
| AtAlaAT1 | 2.4 | ±0.5 | 0.1 | ±0.0 | 2.5 | ±0.4 | 0.1 | ±0.0 |
| AtAlaAT2 | 10.4 | ±1.2 | 1.0 | ±0.1 | 4.9 | ±1.1 | 5.1 | ±0.9 |
| AtGGAT1 | 1.9 | ±0.5 | 0.2 | ±0.1 | 0.2 | ±0.1 | 0.6 | ±0.1 |
| AtGGAT2 | 1.2 | ±0.1 | 8.8 | ±1.8 | 0.2 | ±0.1 | 18.5 | ±2.7 |
| HvAlaAT | 5.6 | ±0.6 | 0.2 | ±0.0 | 4.9 | ±0.5 | 0.1 | ±0.0 |
| MtAlaAT1 | 1.0 | ±0.2 | 0.2 | ±0.0 | 0.1 | ±0.0 | 18.6 | ±2.7 |
| MtAlaAT2 | 1.5 | ±0.2 | 0.3 | ±0.0 | 0.3 | ±0.1 | 18.0 | ±1.9 |
| MmAlaAT1 | 26.5 | ±2.3 | 0.1 | ±0.1 | 13.0 | ±2.4 | 12.5 | ±2.0 |
| PfAlaAT | 4.0 | ±0.5 | 0.02 | ±0.0 | 0.9 | ±0.1 | 16.5 | ±2.2 |
| ScAlaAT1 | 0.3 | ±0.1 | 0.5 | ±0.1 | 0.7 | ±0.1 | 11.0 | ±1.7 |
KM values are shown for each substrate, for each of the ten enzymes examined. Kinetic values represent the average of three independent trials. The correlation coefficient (r2) was >0.80 for all trials, except AtGGAT1 glutamate, AtGGAT2 glutamate and MmAlaAT1 pyruvate. Raw data are plotted in Figure S2.