Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 7;9(2):e1003216. doi: 10.1371/journal.pgen.1003216

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Akiyoshi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Dsn1-S240A,S250A cells are inviable. Serial dilutions (3-fold) of dsn1Δ cells containing DSN1 on a URA3, CEN vector and the indicated integrated point mutants (SBY2318, SBY5948, SBY5949, SBY5950) were plated. Cells that need to maintain the URA3, CEN vector for viability are sensitive to 5-FOA. (B) Dsn1-S240A,S250A protein levels are reduced. Whole cell extracts of Dsn1 and the indicated point mutants (SBY2153, SBY7864, SBY7865, SBY7867) were prepared and analyzed via immunoblot. (C) Overexpression of Dsn1-S240A,S250A restores protein levels. Whole cell extracts of the indicated Dsn1 mutants (SBY8766, SBY8521, SBY7373) were prepared and analyzed via immunoblot. (D) Overexpression of dsn1-S240A,S250A restores viability. Serial dilutions (3-fold) of dsn1Δ strains containing DSN1 on a URA3, CEN vector and a 2 µm LEU2 plasmid with the indicated point mutants (SBY7368, SBY7362, SBY7363, SBY7364 were plated on –ura leu and 5-FOA plates. We did not detect any obvious defect in cells overexpressing wild-type or mutant Dsn1 proteins.