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. 2013 Feb 7;9(2):e1003249. doi: 10.1371/journal.pgen.1003249

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© 2013 Di Bonito et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative ABR measurements of 3 month-old WT, Hoxb1^null and Hoxb1^lateCKO mice. Five ABR peaks are the normal sequential responses evoked by an auditory stimulus at the level of the auditory nerve and along the series of auditory nuclei of the brainstem. The threshold, the lowest intensity of sound at which the response is present, is higher in Hoxb1 mutants than WT mice. (B) Latencies, the time intervals between the stimulus and the diverse response peaks, are normal at different intensities of sound. (C) Mean (± SE) thresholds measured in dB SPL (Sound Pressure Level) for WT, Hoxb1^lateCKO and Hoxb1^null mice at 1, 3, 6, 9 and 12 months of age. The differences between WT, Hoxb1^lateCKO and Hoxb1^null groups are statistically significant at all ages (1 month: WT, n = 24, 41.2±0.5; Hoxb1^null, n = 25, 79.0±2.1; Hoxb1^lateCKO, n = 23, 58.3±2.8; 3 months: WT, n = 28, 41.2±0.4; Hoxb1^null, n = 23, 84.3±1.7; Hoxb1^lateCKO, n = 33, 63.0±2.4; 6 months: WT, n = 22, 45.2±2.5; Hoxb1^null, n = 17, 96.5±2.6; Hoxb1^lateCKO, n = 25, 72.8±2.7; 9 months: WT, n = 20, 51.2±2.5, Hoxb1^null, n = 14, 102.1±3.1, Hoxb1^lateCKO, n = 17, 77.9±3.1; 12 months: WT, n = 10, 56±4, Hoxb1^null, n = 12, 108.9±3.0, Hoxb1^lateCKO, n = 14, 82.1±4.6; ANOVA p<0.001; post hoc t-test p<0.001; WT versus Hoxb1^null; WT versus Hoxb1^lateCKO: p<0.001 for all stages). A progressive increase of hearing thresholds in all groups with age is more prominent in Hoxb1^null and Hoxb1^lateCKO mutants than in the control mice. (D) Mean (± SE) thresholds of ABR for 3-month-old control and Hoxb2^ΔKO mice. The differences between the two groups are statistically significant (WT, n = 5, 52±1.3; Hoxb2^ΔKO, n = 5, 69±5.7; t-test p = 0.013); the threshold increase is similar to that of Hoxb1^lateCKO mice at the same age. (E) ABR of 1 month-old WT and Hoxb1^null mice put in an acoustically isolated environment from birth. Auditory thresholds are increased (WT: 48.9±0.2; Hoxb1^null: 84.2±0.4; p<0,01) similarly to those of non-acoustically isolated mutants (C). dB, decibel.