Figure 3. Genomic changes and drug sensitivities of atovaquone (ATQ)-selected clones.

A. Amino acid change (nucleotide change) and codon position in cytochrome b of each clone. B. Location of the amplification event detected on chromosome 1 for R5a (423,658–643,292 bp). The location of the multidrug resistance associated protein 1 (Pfmrp1, PFA0590w) is shown. The log2 ratio of the intensity of each unique probe in R5a relative to that in S1c is plotted and colored by the moving average over a 500-base pair window. C. The EC₅₀ values for four tested drugs are shown for the 3D7 parent, the sensitive clones and the ATQ-resistant clones of the first generation (G1). Statistically significant differences between the EC₅₀ values of the parental 3D7 line and the ATQ-resistant clones were calculated by a one-way ANOVA followed by a Dunnett posttest (*, p<0.0001). EC₅₀ values are means ± SD of three independent experiments performed in quadruplicate.