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. 2013 Feb 7;9(2):e1003293. doi: 10.1371/journal.pgen.1003293

Figure 4. Detection of deletions in subtelomeric regions by WGS and microarray.

Figure 4

A. Microarray and WGS detection of deletion events. The top two panels show the number of WGS paired-end reads mapping to 16 kb of chromosome 2 for 3D7 and S1a. The third panel shows the same region but with data from the microarray. The log2 ratio of the intensity of each unique probe for S1a relative to 3D7 parent is indicated and colored by the moving average over a 500-base pair window. B. Southern blot analysis. The gDNA of the 3D7 parent and S1a was cut with restriction enzymes HpaI and FspI and analyzed by pulsed field gel electrophoresis using a probe to the rifin gene PFB0015c adjacent to the var gene containing the deletion (schematic on the left, southern blot on the right). Arrows indicate the expected sizes for the fragments of the full-length 3D7 and the truncated S1a var gene (PFB0010w). Stars show nonspecific bands.