Skip to main content
. 2013 Feb 7;9(2):e1003276. doi: 10.1371/journal.pgen.1003276

Figure 4. Loss of cku-80 restores RAD-51 recruitment to meiotic DSBs in com-1 mutant germlines.

Figure 4

(A) Left: representative image of mid-pachytene nuclei in wild-type germlines stained with RAD-51 antibody; merge of RAD-51 (red) and DAPI signal (blue); Right: schematic overview of the C. elegans germline with indicated zones (1–7) used for RAD-51 foci analysis. (B)(C)(D) RAD-51 foci analysis of cku-80, com-1 and com-1 cku-80 double mutant germlines, respectively. Left: representative images of mid-pachytene nuclei (zone 5) stained with RAD-51 antibody, merge of RAD-51 (red) and DAPI signal (blue) Right: Stacked histograms depict the quantification of RAD-51 foci in germlines of the indicated genotypes. The number of RAD-51 foci per nucleus is categorized by the color code shown on the right. The percent of nuclei observed for each category (y-axis) are depicted for each zone along the germline axis (x-axis). Three independent gonads were scored for each genotype. Scale bars, 5 µm.