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. 2013 Feb 7;9(2):e1003276. doi: 10.1371/journal.pgen.1003276

Figure 6. EXO-1 is required for meiotic recombination in absence of COM-1.

Figure 6

(A) Representative image of diplotene nuclei of com-1 cku-80 exo-1 triple mutant animals that express a ZHP-3::GFP transgene (left: GFP signal only, right: merge of GFP and DAPI signal) (B) Representative picture of a diakinesis nucleus in com-1 cku-80 exo-1 triple mutants germlines (C) RAD-51 immunostaining of mid-pachytene nuclei (zone 5) in com-1 cku-80 exo-1 mutant germlines; merge of RAD-51 (red) and DAPI signal (blue) (D) Percentage progeny survival of animals of the indicated genotype; values are the average of 3 independent experiments*, error bars represent S.E.M. (E) Frequency distribution of DAPI-stained entities at diakinesis*. n = number of germlines analyzed. The com-1 cku-80 exo-1 triple mutants occasionally showed >12 DAPI bodies due to chromosomal fragmentation. See Figure 7E for quantification. Scale bars, 5 µm. *These experiments were performed in parallel to those depicted in Figure 1B and 1D; reference values are depicted again here.