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. 2013 Feb 7;9(2):e1003276. doi: 10.1371/journal.pgen.1003276

Figure 7. EXO-1 and COM-1 are needed for efficient interhomolog HR, but dispensable for intersister HR.

Figure 7

(A,B) RAD-51 foci analysis of com-1 cku-80 double and com-1 cku-80 exo-1 triple mutant germlines, respectively. Stacked histograms depict the quantification of RAD-51 foci in germlines of the indicated genotypes. See Figure 4 for details. Representative images of mid-pachytene nuclei (zone 5) and late pachytene nuclei (zone 7) stained with RAD-51 antibody (red). IH = interhomolog, IS = intersister (C) Two representative pictures of diakinesis nuclei of animals of the indicated genotype. White arrows point out chromosomal fragments (D) Representative picture of a diakinesis nucleus of com-1 cku-80 exo-1 triple mutant animals, which are fed on E. coli strains carrying either a control- or rec-8 RNAi vector. (E) Percentage of diakinesis nuclei that show chromosomal fragments; n = number of germlines analyzed. *The difference between these genotypes was highly significant (p<0.001 by Fisher's exact test, two tailed). Scale bars, 5 µm.