Pancreatic tissue from wildtype, Neurod1Δpanc, Nkx2.2null, and Nkx2.2null;Neurod1Δpanc was analyzed by immunofluorescence for expression of the islet hormones glucagon (gluc), ghrelin (ghr) and insulin (ins) at P0 (A–L; white bar indicates 50 microns; DAPI marks all nuclei). Amylase (amyl) expression marks exocrine tissue in all genotypes (A–D). The quantitative expression of glucagon (Gcg) (M), ghrelin (Ghr) (N), and pancreatic polypeptide (Ppy) (O), as well as deletion of Neurod1 (P) was determined by real time PCR using RNA extracted from wildtype, Neurod1Δpanc, Nkx2.2null, Nkx2.2null;Neurod1Δpanc, and Nkx2.2null;Neurod1null pancreas (P0; N = 3–8). Relative mRNA expression was normalized to the housekeeping gene, cyclophilinB. Data are represented as mean+/−SEM. * p<0.05; ** p<0.01; *** p<0.001.