Figure 5. Alpha cells are not rescued with deletion of Neurod1 in glucagon+ cells, in the absence of Nkx2.2.

Pancreatic tissue from wildtype, Neurod1^Δalpha, Nkx2.2^null, and Nkx2.2^null;Neurod1^Δalpha was analyzed by immunofluorescence for expression of the islet hormones glucagon (gluc), ghrelin (ghr) and insulin (ins) at P0 (A–L; white bar indicates 50 microns; DAPI marks all nuclei). Amylase (amyl) expression marks exocrine tissue in all genotypes (A–D). The quantitative expression of glucagon (Gcg) (M), ghrelin (Ghr) (N), and pancreatic polypeptide (Ppy) (O) was determined by real time PCR using RNA extracted from pancreas (P0; N = 3–7). Relative mRNA expression was normalized to the housekeeping gene, cyclophilinB. Data are represented as mean+/−SEM. * p<0.05; ** p<0.01; *** p<0.001.