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. Author manuscript; available in PMC: 2013 Dec 4.
Published in final edited form as: Biochemistry. 2012 Nov 15;51(48):9595–9602. doi: 10.1021/bi301413y

Figure 1.

Figure 1

Aliphatic regions of 2D 1H-13C HSQC spectra of 1 mM [U-13C, 15N-Pro]-IscU acquired at 600 MHz (1H) under the conditions specified. (a) Spectrum of apo-IscU acquired at pH 8.0 and 25 °C, where the protein is a mixture of the S- and D-states. (b) Spectrum of apo-IscU acquired at 45 °C, where the protein is in the D-state. (c) Spectrum of the Zn-bound form of IscU acquired at pH 8.0 and 25 °C, where the protein is in the S-state. Assignments to individual prolyl residues were determined as described in the text. Each NMR sample in a and c contained 1 mM [U-13C, 15N-Pro]-IscU, 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 5 mM DTT, 150 mM NaCl, 50 μM DSS, and 50 μM NaN3 in 10% D2O. The NMR sample in b contained 1 mM [U-13C, 15N-Pro]-IscU:Zn2+, 50 mM Tris-HCl (pH 8.0), 5 mM DTT, 150 mM NaCl, 50 μM DSS, and 50 μM NaN3 in 10% D2O. The same data at a lower contour is shown in Figure S1.