Table 1.

T m analyses, kinetic rate constants, and equilibrium affinities for the different hairpin PAs with their cognate DNA sequence.

	KA1002	KA1007	KA1055	KA1039
ΔTm ± 0.5 °C	6.8	0.6	5.3	3.2
ka (×106 M−1s−1)	44 ± 15	5 ± 2	5 ± 2	3 ± 1.5
kd (×10−3 s−1)	12 ± 2	170 ± 10	6 ± 1	21 ± 5
KA (×108 M−1)	37 ± 7	0.29 ± 0.01 0.27 ± 0.02§	12 ± 2	1.4 ± 0.2
KD (nM)	0.3 ± 0.1	35 ± 3	0.9 ± 0.2	7.1 ± 0.7

^*The errors in these values are based on experimental reproducibility.

^§ Only KA1007 could the K_A value be determined by both kinetic (top) and steady-state (bottom) fitting using a single-site binding model (Materials and Methods).