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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Biochemistry. 2012 Nov 28;51(49):9796–9806. doi: 10.1021/bi301327v

Table 2.

T m analyses for KA1002 and KA1055 with TGGCTT and five mutant sequences.

Cognate and mutant DNA sequences Tm (°C)
Free DNA		 ΔTm* (°C)
KA1002		 ΔTm* (°C)
KA1055
TGGCTT 74.0 6.8 5.3
1 TGCCTT 74.1 11.3 6.2
2 TGTCTT 68.2 4.5 3.4
3 TGGGTT 73.5 0.7 0.6
4 TGGCCT 78.8 3.1 1.3
5 TGATTT 65.6 0.5 0.8
*

The error of these ΔTm values is ± 0.5 °C, based on experimental reproducibility.