Table 2.
Cognate and mutant DNA sequences |
Tm (°C) Free DNA |
ΔTm* (°C) KA1002 |
ΔTm* (°C) KA1055 |
|
---|---|---|---|---|
TGGCTT | 74.0 | 6.8 | 5.3 | |
1 | TGCCTT | 74.1 | 11.3 | 6.2 |
2 | TGTCTT | 68.2 | 4.5 | 3.4 |
3 | TGGGTT | 73.5 | 0.7 | 0.6 |
4 | TGGCCT | 78.8 | 3.1 | 1.3 |
5 | TGATTT | 65.6 | 0.5 | 0.8 |
The error of these ΔTm values is ± 0.5 °C, based on experimental reproducibility.