Skip to main content
. Author manuscript; available in PMC: 2013 Feb 8.
Published in final edited form as: Biochemistry. 2012 Nov 1;51(45):9104–9111. doi: 10.1021/bi301296y

Figure 3.

Figure 3

Temperature-dependent FTIR spectra. (A) FTIR difference spectra in 8 °C increments, generated by subtracting the lowest-temperature spectrum (9 °C) from higher-temperature spectra. The arrow shows the direction of increasing temperature. (B) D spectral components with the corresponding FT temperature profiles (shown as an inset) determined via SVD and global fitting analysis of the temperature-dependent FTIR difference spectra. The first spectral component (—) has a more cooperative transition with an apparent Tm of 66 °C (■), whereas the second spectral component (---) has a broader transition with a lower apparent Tm (□).