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. Author manuscript; available in PMC: 2013 Feb 8.
Published in final edited form as: Biochemistry. 2012 Nov 1;51(45):9104–9111. doi: 10.1021/bi301296y

Figure 4.

Figure 4

Comparison of the wavelength-dependent time-resolved IR measurements with the static FTIR difference spectra. (A) IR transient at 1634 cm–1 of WT CspA in response to a T-jump from 60 to 77 (±1.0) °C. The relaxation is modeled well by a double-exponential fit (—). (B) Time-resolved IR spectra at 50 μs (○) and 1 ms (●) obtained from the frequency-dependent amplitudes of the T-jump transients. The transient spectra are mapped onto the static FTIR difference spectrum [ΔT = 77 – 60 °C (—)].