Cells were grown in LB at 30°C. Representative growth curves are shown with a dashed line in each panel.γ-galactosidase values are averages of at least two independent experiments.
A. Effect of csrA on expression of a plasmid-borne flhDC'-'lacZ translational fusion. Symbols are: WT, black circles; csrA, red squares.
B. Effect of csrA on expression of a chromosomally integrated flhD'-'lacZ translational fusion. Symbols are: WT, black circles; csrA, red squares.
C. Effect of CsrA binding sites on expression of chromosomally integrated flhD'-'lacZ translational fusions in WT (csrA+) strains. Symbols are: WT, black circles; ΔBS1, red squares;ΔBS2, blue diamonds; ΔBS1 ΔBS2, grey triangles.
D. Effect of CsrA binding sites on expression of chromosomally integrated flhD'-'lacZ translational fusions in csrA mutant strains. Symbols are: WT, black circles; ΔBS1, red squares;ΔBS2, blue diamonds; ΔBS1 ΔBS2, grey triangles.