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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Mol Microbiol. 2013 Jan 11;87(4):851–866. doi: 10.1111/mmi.12136

Fig. 7. Primer extension analysis of RNase E cleavage sites in flhDC leader RNA.

Fig. 7

RNA was isolated from pnp rnb and pnp rnb rne mutant strains containing pCSB81 (flhDC) at 0, 1 or 5 min after a shift to the non-permissive temperature (44°C). A primer specific for lacZ was annealed to total cellular RNA and extended by reverse transcriptase. Positions of flhDC leader RNA 5' ends are marked on the right. DNA sequencing ladder is shown.