Table 1.
E. coli strains used in this study
| Strain | Descriptiona | Source |
| C43(DE3) | F− ompT hsdSB (rB- mB-) gal dcm (DE3) | Lucigen |
| CF7789 | F− λ−ΔlacI-lacZ (MluI) | Wei et al, 2001 |
| MG1655 | Prototrophic F- λ- | M. Cashel |
| PLB441 | MG1655/flhDldr::kan | This study |
| PLB442 | CF7789 uvrY::cam flhDldr::kan | This study |
| PLB931 | CF7789/pFDCZ6 (flhDC'-'lacZ) Apr | This study |
| PLB932 | CF7789/csrA::kan pFDCZ6 (flhDC'-'lacZ) Apr | This study |
| PLB966 | CF7789/uvrY::cam csrA::kan | This study |
| PLB1258 | CF7789/flhD'-'lacZ Apr | This study |
| PLB1259 | CF7789/ΔBS1 flhD'-'lacZ Apr | This study |
| PLB1260 | CF7789/ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1262 | CF7789/ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1263 | CF7789/csrA::kan flhD'-'lacZ Apr | This study |
| PLB1264 | CF7789/csrA::kan ΔBS1 flhD'-'lacZ Apr | This study |
| PLB1265 | CF7789/csrA::kan ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1266 | CF7789/csrA::kan ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1601 | CF7789/uvrY::cam rppH::kan | This study |
| PLB1618 | CF7789/uvrY::cam csrA::kan pCSB81 Apr | This study |
| PLB1619 | CF7789/uvrY::cam rppH::kan pCSB81 Apr | This study |
| PLB1620 | CF7789/uvrY::cam pCSB81 Apr | This study |
| PLB1625 | CF7789/uvrY::cam pCSB83 Apr | This study |
| PLB1626 | CF7789/uvrY::cam rppH::kan pCSB83 Apr | This study |
| PLB1627 | CF7789/uvrY::cam csrA::kan pCSB83 Apr | This study |
| PLB1646 | SK6632/pCSB81 (flhDC) Apr | This study |
| PLB1647 | SK6640/pCSB81 (flhDC) Apr | This study |
| PLB1759 | CF7789/ΔE flhD'-'lacZ Apr | This study |
| PLB1760 | CF7789/csrA::kan ΔE flhD'-'lacZ Apr | This study |
| PLB1762 | CF7789/rppH::kan | This study |
| PLB1768 | CF7789/uvrY::cam flhD'-'lacZ Apr | This study |
| PLB1770 | CF7789/uvrY::cam ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1771 | CF7789/uvrY::cam ΔE flhD'-'lacZ Apr | This study |
| PLB1773 | CF7789/uvrY::cam rppH::kan flhD'-'lacZ Apr | This study |
| PLB1774 | CF7789/uvrY::cam rppH::kan ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1775 | CF7789/uvrY::cam rppH::kan ΔE flhD'-'lacZ Apr | This study |
| PLB1776 | CF7789/rppH::kan flhD'-'lacZ Apr | This study |
| PLB1777 | CF7789/rppH::kan ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1778 | CF7789/rppH::kan ΔE flhD'-'lacZ Apr | This study |
| PLB1812 | CF7789/uvrY::cam pyrC::Tn10 flhD'-'lacZ Apr | This study |
| PLB1813 | CF7789/uvrY::cam pyrC::Tn10 ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1814 | CF7789/uvrY::cam pyrC::Tn10 ΔE flhD'-'lacZ Apr | This study |
| PLB1816 | CF7789/uvrY::cam rne-1 flhD'-'lacZ Apr | This study |
| PLB1817 | CF7789/uvrY::cam rne-1 ΔBS1 ΔBS2 flhD'-'lacZ Apr | This study |
| PLB1818 | CF7789/uvrY::cam rne-1 ΔE flhD'-'lacZ Apr | This study |
| PLB1832 | CF7789/uvrY::cam ΔBS1 ΔBS2 ΔE flhD'-'lacZ Apr | This study |
| PLB1845 | CF7789/uvrY::cam pyrC::Tn10 ΔBS1 ΔBS2 ΔE flhD'-'lacZ Apr | This study |
| PLB1846 | CF7789/uvrY::cam rne-1 ΔBS1 ΔBS2 ΔE flhD'-'lacZ Apr | This study |
| PLB1852 | CF7789/uvrY::cam flhDldr::kan flhD- tR2Apr | This Study |
| PLB1853 | CF7789/uvrY::cam flhDldr::kan ΔBS1 ΔBS2 flhD- tR2 Apr | This Study |
| PLB1854 | CF7789/uvrY::cam flhDldr::kan ΔE flhD- tR2 Apr | This Study |
| PLB1855 | CF7789/uvrY::cam flhDldr::kan ΔBS1 ΔBS2 ΔE flhD- tR2 Apr | This Study |
| PLB1856 | CF7789/uvrY::cam pyrC::Tn10 flhDldr::kan flhD- tR2 Apr | This Study |
| PLB1857 | CF7789/uvrY::cam pyrC::Tn10 flhDldr::kan ΔBS1 ΔBS2 flhD-tR2 Apr | This Study |
| PLB1858 | CF7789/uvrY::cam pyrC::Tn10 flhDldr::kan ΔE flhD- tR0 Apr | This Study |
| PLB1859 | CF7789/uvrY::cam pyrC::Tn10 flhDldr::kan ΔBS1 ΔBS2 ΔE flhD-tR2 Apr | This Study |
| PLB1860 | CF7789/uvrY::cam rne-1 flhDldr::kan flhD- tR2 Apr | This Study |
| PLB1861 | CF7789/uvrY::cam rne-1 flhDldr::kan ΔBS1 ΔBS2 flhD- tR2 Apr | This Study |
| PLB1862 | CF7789/uvrY::cam rne-1 flhDldr::kan ΔE flhD- tR2 Apr | This Study |
| PLB1863 | CF7789/uvrY::rne-1 flhDldr::kan ΔBS1 ΔBS2 Δ E flhD- tR2 Apr | This Study |
| SK4390 | thyA715 rph-1 rppH::kan | Mohanty and Kushner, 2010 |
| SK5664 | pyrC::Tn10 Tcr | Arraiano et al, 1988 |
| SK5665 | rne-1 | Babitzke and Kushner, 1991 |
| SK6632 | thyA715 rnb-500 pnp-200 Cmr | Yancey and Kushner, 1990 |
| SK6640 | thyA715 rnb-500 pnp-200 rne-1 Cmr | Yancey and Kushner, 1990 |
| TRCF7789 | CF7789/csrA::kan | Wei et al, 2001 |
| UYCF7789 | CF7789/uvrY::cam | Suzuki et al, 2002 |
a
All flhD'-'lacZ fusions and truncated promoter-leader regions were integrated into the λ att site via the CRIM system (Haldimann and Wanner, 2001). flhDldr::kan indicates that the chromosomal flhDC leader (−238 to +43 with respect to the start of flhD translation) was deleted. ΔBS1 corresponds to a deletion of G7, G8 and A9 from CsrA binding site 1, while ΔBS2 corresponds to a deletion of G50, G51 and A52 from CsrA binding site 2. The ΔBS1 BS2 fusion has all six of these nucleotides deleted. ΔE corresponds to a deletion of several RNase E cleavage sites between +97 to +122 of the flhDC leader. tR2 is an intrinsic terminator from bacteriophage λ.