Figure 5. Induction of β-galactosidase expression in the cerebellum by Cre recombinase.
(A) Purkinje cell-specific staining was observed in totteringPC-loss (Cacna1atg/flox mice carrying the Purkinje cell specific L7-Cre transgene) that also carried the Rosa26 Cre reporter allele. There was no obvious Purkinje cell loss in these mice. (B) No staining was observed in Cacna1atg/flox mice carrying the Rosa26 Cre reporter allele but not the L7-Cre transgene. (C) Cacna1atg/flox mice carrying the Rosa26 Cre reporter allele treated with intracerebellar injections of Cre-lentivirus revealed induction of β-galactosidase expression near the injection site. (D) Staining was absent in sections distant from the Cre-lentivirus injection site. Scale bar = 100 μm.